NaCl improves siRNA delivery mediated by nanoparticles of hydroxyethylated cationic cholesterol with amido-linker

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• 作者：Yoshiyuki Hattori ; Ayako Hagiwara ; Wuxiao Ding ; Yoshie Maitani
• 关键词：Cationic nanoparticles ; Synthetic siRNA ; siRNA delivery ; Transfection ; Nanoplex ; PC-3 cells ; Caveolae-mediated endocytosis ; Membrane fusion
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2008
• 出版时间：1 October 2008
• 年：2008
• 卷：18
• 期：19
• 页码：5228-5232
• 全文大小：391 K

文摘

We investigated the effect of amido- (NP-OH) and carbamate linkers (NP-HAPC) in nanoparticles composed of hydroxyethylated cationic cholesterol on siRNA transfection. The presence of NaCl in forming a NP-OH nanoplex increased the suppressive effect of gene expression by increasing the size of the nanoplex and changing the cellular uptake mechanism from membrane fusion and clathrin-mediated endocytosis to clathrin- and caveolae-mediated endocytosis.