The Phytotherapeutic Agent, Eviprostat, Suppresses Stromal Proliferation and Inflammation Even After Establishment of Nonbacterial Prostatitis in the Rat Prostate

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• 作者：Shinsuke Shibuya^a ; Zhang Xia^b ; Mikio Sugimoto^b ; Nobufumi Ueda^b ; Reiji Haba^a ; Yoshiyuki Kakehi^b ; ^{kakehi@med.kagawa-u.ac.jp" class="auth_mail}
• 刊名：Urology
• 出版年：March, 2014
• 年：2014
• 卷：83
• 期：3
• 页码：528-534
• 全文大小：255 K

文摘

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Objective

To evaluate the effect of phytotherapeutic agent, Eviprostat, administered after the establishment of nonbacterial prostatitis (NBP) on the stroma-to-epithelium ratio (S/E ratio), inflammatory scores, tissue macrophage infiltration, and cytokines and chemokines levels in prostate tissue and urine.

Materials and Methods

Ten-month-old male Wistar rats were castrated and exposed to 17-beta-isomer of estradiol for 30 days to induce NBP. Twenty-five NBP rats were divided into 5 groups: (1) NBP (0) rats sacrificed immediately after the establishment of NBP; (2,3) NBP (30)/control (CTL) and NBP (30)/Eviprostat (EVI) rats fed without or with 0.1% Eviprostat under estradiol-free for 30 days, respectively; and (4,5) NBP (60)/CTL and NBP (60)/EVI rats fed without or with 0.1% Eviprostat under estradiol-free for 60 days, respectively. The S/E ratio, inflammatory scores, and the number of macrophage infiltration in the prostate were assessed. Concentrations of cytokines and chemokines in prostatic tissue and urine were measured by enzyme-linked immunosorbent assay.

Results

The S/E ratio was significantly increased with time until 60 days under estradiol-free condition (P聽<.001). The S/E ratio and the inflammatory scores in NBP (60)/EVI was significantly lower than that of NBP (60)/CTL (P聽<.001, and P聽= .022, respectively). The mean tissue concentration of chemokine ligand 2 (CCL2)/monocyte chemoattractant protein-1 (MCP-1) in NBP (60)/CTL was significantly higher than that in NBP (0) (P聽= .016), whereas, there was no difference between NBP (60)/EVI and NBP (0). Furthermore, urinary CCL2/MCP-1 was significantly decreased in NBP (60)/EVI as compared with NBP (0) (P聽= .028).

Conclusion

Eviprostat suppresses the stromal proliferation and inflammation in the rat prostate after the establishment of NBP at least partly owing to inhibitory effect on CCL2/MCP-1 production in the prostate.