Endogenous abscisic acid is involved in methyl jasmonate-induced reactive oxygen species and nitric oxide production but not in cytosolic alkalization in Arabidopsis guard cells

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• 作者：Wenxiu Ye ; Mohammad Anowar Hossain ; Shintaro Munemasa ; Yoshimasa Nakamura ; Izumi C. Mori ; Yoshiyuki Murata
• 关键词：ABA ; abscisic acid ; BCECF-AM ; 2&prime ; 7&prime ; -bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester ; [Ca2+]cyt ; cytosolic free calcium concentration ; DAF-2DA ; 4 ; 5-diaminofluorescein-2 diacetate ; FLU ; fluridon ; H2DCF-DA ; 2&prime ; 7&prime ; -dichl
• 刊名：Journal of Plant Physiology
• 出版年：2013
• 出版时间：1 September, 2013
• 年：2013
• 卷：170
• 期：13
• 页码：1212-1215
• 全文大小：516 K

文摘

We recently demonstrated that endogenous abscisic acid (ABA) is involved in methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we investigated whether endogenous ABA is involved in MeJA-induced reactive oxygen species (ROS) and nitric oxide (NO) production and cytosolic alkalization in guard cells using an ABA-deficient Arabidopsis mutant, aba2-2, and an inhibitor of ABA biosynthesis, fluridon (FLU). The aba2-2 mutation impaired MeJA-induced ROS and NO production. FLU inhibited MeJA-induced ROS production in wild-type guard cells. Pretreatment with 0.1 ¦ÌM ABA, which does not induce stomatal closure in the wild type, complemented the insensitivity to MeJA of the aba2-2 mutant. However, MeJA induced cytosolic alkalization in both wild-type and aba2-2 guard cells. These results suggest that endogenous ABA is involved in MeJA-induced ROS and NO production but not in MeJA-induced cytosolic alkalization in Arabidopsis guard cells.