Electrochemical immunosensor based on Pd-Au nanoparticles supported on functionalized PDDA-MWCNT nanocomposites for aflatoxin B1 detection

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• 作者：Songbai Zhang^a ; ^b ; Youming Shen^a ; Guangyu Shen^a ; ^{sgyrab@163.com" class="auth_mail" title="E-mail the corresponding author} ; Sha Wang^a ; Guoli Shen^b ; Ruqin Yu^b
• 关键词：Carbon nanotubes ; Poly(diallyldimethylammoniumchloride) ; Pd&ndash ; Au nanoparticles ; Aflatoxin B1 ; Immunosensors
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：1 February 2016
• 年：2016
• 卷：494
• 期：Complete
• 页码：10-15
• 全文大小：926 K

文摘

This paper reports a label-free electrochemical immunosensor for the determination of aflatoxin B1 (AFB1), which is based on a gold electrode modified by a biocompatible film of carbon nanotubes/poly(diallyldimethylammoniumchloride)/Pd–Au nanoparticles (CNTs/PDDA/Pd–Au). The nanocomposite was characterized by transmission electron microscopy and the electrochemical behavior of modified electrodes was investigated by cyclic voltammetry. The CNTs/PDDA/Pd–Au nanocomposites film showed good electron transfer ability, which ensured high sensitivity to detect AFB1 in a range from 0.05 to 25 ng mL⁻¹ with a detection limit of 0.03 ng mL⁻¹ obtained at 3σ (where σ is the standard deviation of the blank solution, n = 10). The proposed immunosensor provides a simple tool for AFB1 detection. This strategy can be extended to any other antigen detection by using the corresponding antibodies.