Oral pirfenidone protects against fibrosis by inhibiting fibroblast proliferation and TGF-β signaling in a murine colitis model

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• 作者：Guanwei Li^a ; Jianan Ren^a ; ^{jiananr@smail.nju.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Qiongyuan Hu^a ; ^b ; Youming Deng^a ; Guopu Chen^a ; Kun Guo^a ; Ranran Li^a ; Yuan Li^a ; Lei Wu^a ; Gefei Wang^a ; Guosheng Gu^a ; Jieshou Li^a
• 关键词：Pirfenidone ; Inflammatory bowel disease ; Intestinal fibrosis ; Antifibrotic therapy
• 刊名：Biochemical Pharmacology
• 出版年：2016
• 出版时间：1 October 2016
• 年：2016
• 卷：117
• 期：Complete
• 页码：57-67
• 全文大小：5772 K

文摘

Inflammatory bowel disease (IBD), particularly Crohn’s disease, frequently causes intestinal fibrosis that ultimately leads to formation of strictures requiring bowel resection. Currently there is no effective antifibrotic therapy available for this disease. Pirfenidone is a small compound that has a broad spectrum of antifibrogenic effect and has been used for the treatment of fibrotic diseases in various organs. The present study aimed to investigate the antifibrogenic effect of pirfenidone in a dextran sulfate sodium (DSS)-induced murine colitis model. C57BL/6 mice were used and animals were randomly divided into groups receiving pirfenidone or vehicle by oral or transanal routes. Inflammation- and fibrosis-related indexes including body weight, colon length, disease activity, histological change, mRNA expression of pro-inflammatory and pro-fibrogenic cytokines were assessed. Furthermore, we performed in vitro analysis using CCD18-Co fibroblasts to evaluate cell proliferation, transdifferentiation, and viability after the cells were cultured with pirfenidone. It was found that oral administration of pirfenidone reduced deposition of collagen in colitis-associated fibrosis, and significantly suppressed the mRNA expression of col1a2, col3a1, and TGF-β. Moreover, pirfenidone inhibited the activation of TGF-β-related smad and MAPK pathways both in vitro and in vivo. Clinical and histological evaluation demonstrated that pirfenidone had no anti-inflammatory effect. The antifibrogenic effect was reduced when pirfenidone was administered in a delayed manner and was unobserved if given locally. Pirfenidone suppressed fibroblast proliferation and transdifferentiation without observed toxicity. Altogether, our results suggested that oral pirfenidone protects against fibrosis of DSS-induced colitis through inhibiting the proliferation of colonic fibroblasts and TGF-β signaling pathways.