Matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of peptide citrullination site using Br signature

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• 作者：Mikyung Choi^a ; Jin-Su Song^a ; ¹ ; Hie-Joon Kim^a ; ^{hjkim1@snu.ac.kr} ; Sangwon Cha^b ; Eun Young Lee^c
• 关键词：Citrullination ; MALDI-TOF MS ; 4-Bromophenyl glyoxal
• 刊名：Analytical Biochemistry
• 出版年：2013
• 出版时间：1 June, 2013
• 年：2013
• 卷：437
• 期：1
• 页码：62-67
• 全文大小：833 K

文摘

Enzymatic conversion of arginine to citrulline by peptidyl arginine deiminase is associated with peptide presentation and development of autoimmunity in rheumatoid arthritis. In order to facilitate identification of the citrullination site, citrulline residue was modified using 4-bromophenyl glyoxal, and 194 Da mass increase and incorporation of the Br signature were confirmed by MALDI-TOF MS. Using this approach, we identified four and five citrullination sites of bovine serum albumin and bovine fibrinogen, respectively. MALDI-TOF/TOF MS was used to unambiguously identify two citrullination sites from bovine fibrinogen.