PLGA-GC core-shell microspheres were fabricated for delivering two model drugs in sequence. The CHA loaded in the shell was rapidly released, and the proteins at the core exhibited a sustained release. The released CHA and bFGF retained their antimicrobial activity and bioactivity. The microspheres exhibited non-cytotoxicity and had a good biocompatibility. This sequential delivery system has the potential for preventing infection and promoting wound healing.