Effects of in-vitro cultured calculus bovis on learning and memory impairments of hyperlipemia vascular dementia rats

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• 作者：Xiao-Ming Zhong^a ; ^{k6_zxm@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Xue-Cong Ren^b ; ^{rxc880715@163.com" class="auth_mail" title="E-mail the corresponding author} ; Ye-Liang Lou^a ; ^{carllouye@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Meng-Jing Chen^a ; ^{xin121245@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Guan-Ze Li^a ; ^{li_guanze@163.com" class="auth_mail" title="E-mail the corresponding author} ; Xue-Yuan Gong^a ; ^{gongxue_yuan123@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Zhen Huang^a ; ^{huangzhen@zcmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ICCB ; in-vitro cultured calculus bovis ; HVD ; hyperlipemia vascular dementia ; UV&ndash ; vis ; ultraviolet&ndash ; visible spectrophotometry ; VD ; Vascular Dementia ; TCM ; Traditional Chinese Medical ; HCD ; high cholesterol diet ; MWMT ; Morris water maze test ; ELT ; escape latency time ; SS ; searching strategies ; RSS ; random searching strategy ; TSS ; tendency searching strategy ; SSS ; straight searching strategy ; TCP ; times of crossing platform ; PSPQ ; percentage of swimming at platform quadrant ; SBT ; shuttle
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 November 2016
• 年：2016
• 卷：192
• 期：Complete
• 页码：390-397
• 全文大小：1829 K

文摘

In-vitro cultured calculus bovis (ICCB) is a quality substitute for natural bezoar which is used for the therapeutic purpose of treating encephalopathy. ICCB has been authorized to use on clinic. The aim of the study is to evaluate the effects and the potential mechanisms of in-vitro cultured calculus bovis (ICCB) on learning and memory impairments of hyperlipemia vascular dementia (HVD) rats.

Materials and methods

The HVD model was established by permanent occlusion of bilateral common carotid arteries based on hyperlipemia rats. Learning and memory abilities were evaluated by morris water maze test and shuttle box test. Ultraviolet–visible spectrophotometry (UV–vis) was employed to determine the SOD, MDA and NO in cerebral tissue, as well as the TG in serum. HE staining and toluidine blue staining were employed to evaluate cone cells damage in hippocampus CA1. An immunohistochemistry was used to measure the Bax and Bcl-2 expressions in cerebral tissue.

Results

Compared with control group, the abilities of spatial learning and memory and conditional memory were decreased significantly in HVD group (P<0.01, P<0.05). MDA content in cerebral tissue was remarkably increased while the SOD activity and NO content were both decreased (P<0.01). TG content in serum was increased remarkably (P<0.01). And the cone cells in hippocampus CA1 were damaged obviously. Compared with HVD group, ICCB treatment improved the abilities of learning and memory, elevated the SOD activity (P<0.01, P<0.05), reduced the MDA content (P<0.01) as well as the TG content in serum (P<0.01), increased the NO content (P<0.01), improved the damaged cone cells in hippocampus CA1, increased the number of cones cells (P<0.01), decreased the Bax expression, and increased the Bcl-2 expression (P<0.01).

Conclusion

ICCB could improve the abilities of learning and memory in HVD rats. It might be related to anti-oxidative, regulation of Bax and Bcl-2 expressions, and the alleviation of cone cells damage.