In vitro evaluation of inorganic and methyl mercury mediated cytotoxic effect on neural cells derived from different animal species

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• 作者：Jing Tong¹ ; ³ ; Youwei Wang¹ ; Yuanan Lu² ; ³ ; ^{yuanan@hawaii.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：1640 ; RPIM-1640 medium ; BSA ; bovine serum albumin ; BV-2 ; mouse microglia cells ; CB ; Coomassie blue ; CHME-5 ; transformed human microglia cells ; CNS ; central nervous system ; DMEM ; Dulbecco's Modified Eagle's Medium ; DMSO ; dimethyl sulfoxide ; FBS ; fetal bovine serum ; HGST-BR ; healthy green turtle brain cells ; IC50 ; 50% inhibition concentration ; MeHgCl ; methylmercury(II) chloride ; MEM ; minimal essential medium ; MTT ; thiazolyl blue tetrazolium bromide ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyl tetraz
• 刊名：Journal of Environmental Sciences
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：41
• 期：Complete
• 页码：138-145
• 全文大小：257 K

文摘

To extend the current understanding of the mercury-mediated cytotoxic effect, five neural cell lines established from different animal species were comparatively analyzed using three different endpoint bioassays: thiazolyl blue tetrazolium bromide, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT), neutral red uptake assay (NRU), and Coomassie blue assay (CB). Following a 24-hr exposure to selected concentrations of mercury chloride (HgCl₂) and methylmercury (II) chloride (MeHgCl), the cytotoxic effect on test cells was characterized by comparing their 50% inhibition concentration (IC₅₀) values. Experimental results indicated that both these forms of mercury were toxic to all the neural cells, but at very different degrees. The IC₅₀ values of MeHgCl among these cell lines ranged from 1.15 ± 0.22 to 10.31 ± 0.70 μmol/L while the IC₅₀ values for HgCl₂ were much higher, ranging from 6.44 ± 0.36 to 160.97 ± 19.63 μmol/L, indicating the more toxic nature of MeHgCl. The IC₅₀ ratio between HgCl₂ and MeHgCl ranged from 1.75 to 96.0, which confirms that organic mercury is much more toxic to these neural cells than inorganic mercury. Among these cell lines, HGST-BR and TriG44 derived from marine sea turtles showed a significantly high tolerance to HgCl₂ as compared to the three mammalian neural cells. Among these neural cells, SK-N-SH represented the most sensitive cells to both chemical forms of mercury.