Ionic liquids-based microwave-assisted extraction of active components from pigeon pea leaves for quantitative analysis

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• 作者：Zuofu Wei ; Yuangang Zu ; Yujie Fu ; ^{yujie_fu2002@yahoo.com} ; Wei Wang ; Meng Luo ; Chunjian Zhao ; Youzhi Pan
• 关键词：Pigeon pea ; Ionic liquids ; Flavonoids ; Stilbenes ; Coumarins
• 刊名：Separation and Purification Technology
• 出版年：2013
• 出版时间：4 January, 2013
• 年：2013
• 卷：102
• 期：Complete
• 页码：75-81
• 全文大小：957 K

文摘

The present study aims to extract active components from pigeon pea leaves by ionic liquid-based microwave-assisted extraction (ILMAE) for quantitative analysis. Compared with ionic liquid-based heating extraction (ILHE) and ultrasonic-assisted extraction (ILUAE), ILMAE exhibited higher extraction efficiency. A series of 1-alkyl-3-methylimidazolium ILs with different anions and alkyl chain lengths of cations were investigated, and 1-butyl-3-methylimidazolium bromide ([C4MIM]Br) solution was selected as extractants. Subsequently, ILMAE parameters were optimized, and the optimized conditions were [C4MIM]Br concentration 0.75 M, extraction temperature 60 ¡ãC, solvent to solid ratio 12:1 mL/g, and irradiation time 7 min. Under the optimal conditions, the extraction efficiency increased by 11.0-38.9 % compared with ILHR and ILUAE, and the recovery achieved more than 95.3 % . Furthermore, a rapid analysis method, HPLC-DAD with an UPLC column, was established and validated. This method exhibited satisfactory linearity (r² > 0.999), precision (RSD < 4.6 % ) and recovery (97.14-103.38 % ), and was successfully applied for the quantification of nine components in pigeon pea leaves within 25 min. The results obtained provide a fast and safe method for determination of active components in pigeon pea leaves.