In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand

详细信息    查看全文

• 作者：Jun Ai ; Tao Li ; Bingling Li ; Yuanhong Xu ; Dan Li ; Zuojia Liu ; Erkang Wang
• 关键词：AS1411 ; Nucleolin ; HeLa cells ; Fluorescence ; Bioimaging
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 出版时间：5 September, 2012
• 年：2012
• 卷：741
• 期：Complete
• 页码：93-99
• 全文大小：998 K

文摘

In this article, we reported a novel approach for in situ labeling and imaging HeLa cancer cells utilizing a bifunctional aptamer (AS1411) and its fluorescent ligand, protoporphyrin IX (PPIX). In the presence of potassium ion, AS1411 folded to G-quadruplex structure, binded fluorescent ligand (PPIX) with fluorescent enhancement, and targeted the nucleolin overexpressed by cancer cells. Consequently, bioimaging of cancer cells specifically were realized by laser scanning confocal microscope. The bioimaging strategy with AS1411-PPIX complex was capable to distinguish HeLa cancer cells from normal cells unambiguously, and fluorescence imaging of cancer cells was also realized in human serum. Moreover, the bioimaging method was very facile, effective and need not any covalent modification. These results illustrated that the useful approach can provide a novel clue for bioimaging based on non-covalent bifunctional aptamer in clinic diagnosis.