A novel DNA vaccine for protective immunity against virulent Mycobacterium bovis in mice

详细信息    查看全文

• 作者：Siguo Liu ; Qiang Gong ; Chunlai Wang ; Huifang Liu ; Yong Wang ; Sheping Guo ; Weili Wang ; Ji ; ong Liu ; Meili Shao ; Lei Chi ; Kun Zhao ; Zhenguo Wang ; Yuanxiang Shi ; Ying Huang ; Aman guli ; Chunsheng Zhang ; Xi
• 关键词：Mycobacterium bovis ; DNA vaccine ; Immune efficacy
• 刊名：Immunology Letters
• 出版年：2008
• 出版时间：15 May 2008
• 年：2008
• 卷：117
• 期：2
• 页码：136-145
• 全文大小：1156 K

文摘

Four mycobacteria candidate genes (Ag85A, Ag85B, Mpt64 and PstS3) were cloned, fused with ESAT6 and ligated into eukaryotic expression vectors. Combined poly-rBCG and mIL-12 vaccines were transferred into a murine bladder tumor model. The efficiency of gene expression was detected using Western blotting, flow cytometry and semiquantitative reverse transcriptase-polymerase chain reaction. Systemic cytokine responses, tumor growth and cumulative survival rates were monitored.

Results

Transfected bladder cancer cells showed high in vitro and in vivo expression of the recombinant subcomponents. Mice with tumors injected with poly-rBCG plus mIL-12 produced serum interferon-γ significantly within 21 days but no significant elevations in tumor necrosis factor-α, IL-2, IL-4 or IL-5 were found. On day 28 after electroporation the growth of MBT-2 implants treated with poly-rBCG, mIL-12 or poly-rBCG plus mIL-12 was significantly inhibited. The cumulative survival of mice treated with poly-rBCG plus mIL-12 was significantly higher than that of the other 3 groups.

Conclusions

Highly immunopotent recombinant vaccines of bacillus Calmette-Guerin DNA were produced that elicited T helper 1 immune responses with a high serum interferon-γ level, inhibited tumor growth and prolonged the survival of tumor bearing mice. Thus, electroporation immunogene therapy using poly-rBCG plus mIL-12 may be an attractive regimen for the treatment of bladder cancer.