A simple but efficient electrochemical method to assay protein arginine deiminase 4
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- 作者：Xixian ChenaAuthor Vitae ; Yun Lva ; bAuthor Vitae ; Yuanyuan ZhangcAuthor Vitae ; Jing Zhaoa ; jingzhao@shu.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Lizhou Sunc ; lizhou_sun121@hotmail.com" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Protein arginine deiminase 4 ; Electrochemical assay ; Inhibitor screening ; Citrullination ; [Ru(NH3)5Cl]2+
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：227
- 期：Complete
- 页码：43-47
- 全文大小：841 K
文摘
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A simple electrochemical method to assay PAD4 activities and screen its inhibitors.
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Substrate peptides on electrode surface can strongly repel [Ru(NH3)5Cl]2+.
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PAD4-catalyzed citrullination can reduce positive charges on the electrode surface.
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The addition of PAD4 inhibitors can result in low electrochemical response.