Survivin-targeted nanoparticles for pancreatic tumor imaging in mouse model

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• 作者：Zhongqiu Wang ; MD ; PhD^a ; ¹ ; ^{zhq2001us@163.com" class="auth_mail" title="E-mail the corresponding author} ; Mingmin Tong ; MS^b ; ¹ ; Xiao Chen ; PhD^a ; Shouyou Hu ; MD^c ; Zhijian Yang ; PhD^d ; Yu Zhang ; PhD^e ; Hao Zhou ; MS^a ; Yuefei Wu ; MS^f ; Xiaoshuang Li ; MS^a ; Donghui Li ; PhD^g
• 关键词：Survivin ; Antisense oligonucleotide ; Magnetic nanoparticles ; Pancreatic cancer ; Magnetic resonance imaging
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：12
• 期：6
• 页码：1651-1661
• 全文大小：1963 K

文摘

We investigated the potential of targeting survivin, an inhibitor of apoptosis, in visualize pancreatic tumor in mouse model using targeted magnetic nanoparticles (MNPs) and magnetic resonance imaging (MRI). Chitosan-coated MNPS and survivin antisense oligonucleotide(ASON) were conjugated to give Sur-MNPs. Accumulations of targeted, non-targeted nanoparticles or nonsense oligonucleotide-MNPs (NSON-MNPs) in the liver, spleen, kidney and tumors were determined. Targeted nanoparticles were highly accumulated in BxPC-3 cells but not in non-cancer cells. In vivo MRI showed a significant T₂ signal reduction in tumors of mice injected with targeted nanoparticles but slight signal change in tumors of mice injected with non-targeted nanoparticles or NSON-MNPs. Prussian blue staining demonstrated highly accumulated Sur-MNPs in tumor mass compared with normal pancreatic, kidney and liver tissues. Our data show that the MNPs functionalized with ASON lead to the targeted localization in pancreatic tumors. Survivin targeted nanoparticles could be used for detection of pancreatic tumors.