One-pot electrodeposition of 3-aminopropyltriethoxysilane–chitosan hybrid gel film to immobilize glucose oxidase for biosensing

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• 作者：Lihong Lei^a ; ^b ; Zhijun Cao^a ; Qingji Xie^a ; ^{xieqj@hunnu.edu.cn"" rel=""nofollow} ; Yingchun Fu^a ; Yueming Tan^a ; Ming Ma^a ; Shouzhuo Yao^a [Author vitae]
• 关键词：Electrodeposition of 3-aminopropyltriethoxysilane/chitosan hybrid gel film ; Enzyme immobilization ; Glucose biosensing ; Electroreduction of p-benzoquinone ; Electrochemical quartz crystal microbalance
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2011
• 出版时间：20 September 2011
• 年：2011
• 卷：157
• 期：1
• 页码：282-289
• 全文大小：942 K

文摘

We report on the electrodeposition of a 3-aminopropyltriethoxysilane-chitosan (APTES–CS) hybrid gel film for in situ immobilization of glucose oxidase (GOx) on an Au or platinized Au (Pt_nano/Au) electrode for biosensing of glucose. Controllable electroreduction of p-benzoquinone is used to lift the electrode-surface pH for the GOx–APTES–CS codeposition, which was monitored by an electrochemical quartz crystal microbalance. The fabrication procedures of the biosensor and the parameters influencing the biosensing performance were optimized. The prepared porous GOx–APTES–CS/Pt_nano/Au and GOx–APTES–CS/Au electrodes can be used to detect the enzymatically generated H₂O₂ at 0.5 and 0.7 V vs SCE, respectively. The enzyme electrodes exhibited linear responses to glucose concentration from 0.2 μM to 8.2 mM (R = 0.998, at Pt_nano/Au substrate) and from 0.2 μM to 5.5 mM (R = 0.998, at Au substrate), with current sensitivities of 69.5 (Pt_nano/Au) and 65 (Au) μA mM⁻¹ cm⁻², respectively, and a detection limit of 0.2 μM (S/N = 3) was achieved for each electrode. The response time was less than 5 (Pt_nano/Au) or 8 (Au) s. It is striking that the enzyme electrodes remained their initial response sensitivity after storage for 5 (Au) and >6 (Pt_nano/Au) months in 0.10 M PBS (pH 7.0) at 4 °C.