Effective inhibition of HIV-1 replication in cultured cells by external guide sequences and ribonuclease P

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• 作者：Barnor ; Jacob S. ; Endo ; Yumihiko ; Habu ; Yuichiro ; Miyano-Kurosaki ; Naoko ; Kitano ; Michiko ; et. al.
• 关键词：RNase P ; External guide sequence ; HIV-1 ; pol III Promoter ; Gene therapy
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2004
• 出版时间：October 4, 2004
• 年：2004
• 卷：14
• 期：19
• 页码：4941-4944
• 全文大小：352 K

文摘

We examined the suppressive effect of HIV-1 RNA gene cleavge on HIV-1 expression, using the catalytic RNA subunit RNase P and the 3aaa-half tRNA^Try [external guide sequence (EGS)] in cultured cells. HIV-1 expression was inhibited by the tRNA^met-EGS-U5 and U6-EGS-U5 from the tRNA^met and U6 promoters, respectively. There was no difference in the inhibitory effects on HIV-1 expression between the tRNA^met and U6 promoters.