Simple analytic method of diaminopimelate epimerase activity

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• 作者：Hiromi Miura¹ ; Kentaro Hori¹ ; Yosuke Sasaki² ; Yuki Inahashi² ; Yasufumi Yagisawa² ; Nobuyuki Fujita³ ; Satoshi ¨­mura¹ ; Y¨­ko Takahashi¹ ; ² ; ^{ytakaha@lisci.kitasato-u.ac.jp}
• 关键词：Diaminopimelate epimerase ; Preparative thin-layer chromatography ; Diaminopimelate isomers ; High-performance liquid chromatography ; Kitasatospora setae
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：116
• 期：2
• 页码：253-255
• 全文大小：396 K

文摘

A simple method to analyze diaminopimelate epimerase activity has been devised which uses thin-layer chromatography and chiral column chromatography for preparation of pure diaminopimelate isomers and detection of products, respectively.