Spectral editing and pattern recognition methods applied to high-resolution magic-angle spinning ¹H nuclear magnetic resonance spectroscopy of liver tissues

详细信息    查看全文

• 作者：Wang ; Yulan ; Bollard ; Mary E. ; Keun ; Hector ; Antti ; Henrik ; Beckonert ; Olaf ; Ebbels ; Timothy M. ; et. al.
• 关键词：Principal components analysis ; Metabonomics ; HRMAS ¹H NMR spectroscopy ; NMR spectral editing ; Liver tissue ; Hydrazine
• 刊名：Analytical Biochemistry
• 出版年：2003
• 出版时间：December 1, 2003
• 年：2003
• 卷：323
• 期：1
• 页码：26-32
• 全文大小：250 K

文摘

Principal component analysis (PCA) has been applied to three nuclear magnetic resonance (NMR) spectral editing methods, namely, the Carr–Purcell–Meiboom–Gill spin-echo, diffusion editing, and skyline projection of a two-dimensional J-resolved spectrum, obtained from high-resolution magic-angle spinning NMR spectroscopy of liver tissues, to distinguish between control and hydrazine-treated rats. The effects of the toxin on rat liver biochemistry were directly observed and characterized by depleted levels of liver glycogen, choline, taurine, trimethylamine N-oxide, and glucose and by elevated levels of lipids and alanine. The highly unsaturated ω-3-type fatty acid was observed for the first time in hydrazine-treated rat liver. The contributions of the metabolites to the separation of control from dosed liver tissues varied depending on the type of spectral editing method used. We have shown that subtle changes in the metabolic profiles can be selectively amplified using a metabonomics approach based on the different NMR spectral editing techniques in conjunction with PCA.