The global transcriptional landscape of Bacillus amyloliquefaciens XH7 and high-throughput screening of strong promoters based on RNA-seq data
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- 作者：Yuling Liaoa ; b ; Lianggang Huanga ; Bin Wanga ; btbinwang@scut.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Feng Zhoua ; Li Pana ; btlipan@scut.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：RNA-seq ; RNA sequencing ; TSSs ; transcriptional start sites ; RPKM ; reads per kilobase of transcripts per million mapped reads ; UTRs ; untranslated regions ; PGPR ; plant growth-promoting rhizobacterium ; CDSs ; protein-coding sequences ; CGMCC ; China General Microbiological Culture Collection Center ; LB ; Luria-Bertani ; Amp ; ampicillin ; Kan ; kanamycin ; cDNA ; complementary DNA ; GO ; Gene Ontolog ; ORFs ; Open reading frames ; TFs ; transcription factors ; PCR ; Polymerase Chain Reaction ; RBS ; ribosome binding s
- 刊名：Gene
- 出版年：2015
- 出版时间：25 October 2015
- 年：2015
- 卷：571
- 期：2
- 页码：252-262
- 全文大小：1536 K
文摘
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We presented the global transcriptional landscape of B. amyloliquefaciens.
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The transcript structures analysis identified 1064 transcriptional start sites (TSS) and 749 operons.
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The candidate promoter Pr2 (promoter for the sigW gene) exhibited the strongest activity.