Toehold enabling stem-loop inspired hemiduplex probe with enhanced sensitivity and sequence-specific detection of tumor DNA in serum
详细信息 查看全文
- 作者：Fan Yang1 ; Siqi Wang1 ; Yulin Zhang ; Lina Tang ; Dan Jin ; Yong Ning ; ningyong128@163.com" class="auth_mail" title="E-mail the corresponding author ; Guo-Jun Zhang ; zhanggj@hbtcm.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Electrochemical DNA sensor ; Stem-loop structure ; Toehold ; Exonuclease Ⅲ ; Tumor DNA
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 August 2016
- 年：2016
- 卷：82
- 期：Complete
- 页码：32-39
- 全文大小：2322 K
文摘
- •
We develop an ultrasensitive and highly specific electrochemical sensor inspired by stem-loop structured probe.
- •
The sensor interfacial design is compatible with homogeneous enzymatic amplification.
- •
This tE-DNA sensor achieves a high sensitivity up to 0.18 fM.
- •
This sensor is reusable and can be employed directly in 50% human serum with minimal signal degradation.