Serum depletion, multidrug resistance and fluorescent probes: Methodological implications in free radicals evaluation

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• 作者：Ilaria Peluso ; Luca Romanelli ; Silvana Spagnuolo ; Husseen Manafikhi ; Maura Palmery ; ^{maura.palmery@uniroma1.it" class="auth_mail}
• 关键词：Intracellular probes ; Multidrug resistance ; Oxidative stress ; Quercetin
• 刊名：Journal of Immunological Methods
• 出版年：March, 2014
• 年：2014
• 卷：405
• 期：Complete
• 页码：178-180
• 全文大小：310 K

文摘

Intracellular probes used for oxidative burst evaluation could be a substrate of the multidrug resistance proteins (MDR), which may cause misinterpretation of experimental data. We aimed to study the effect of the culture condition and of the MDR-interfering antioxidant quercetin on free radical measurement in serum depleted HCT-8 cells and chick embryo hepatocytes. Serum depletion and/or quercetin affected the traffic of 2鈥?7鈥?bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) and calcein. The comparative results obtained with 2鈥?7鈥?dichlorofluorescein (DCF) and with the plasma-membrane probe C11-BODIPY 581/591 [4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid], suggest caution in the use of intracellular probes to evaluate oxidative stress in vitro.