Antitumor activity of Annona squamosa seed oil

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• 作者：Yong Chen ; ^{achenyong@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Yayun Chen ; Yeye Shi ; Chengyao Ma ; Xunan Wang ; Yue Li ; Yunjie Miao ; Jianwei Chen ; Xiang Li ; ^{lixiang_8182@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Annona squamosa seed oil ; Soxhlet extraction ; Unsaturated fatty acid ; Annonaceous acetogenin ; Anti-tumor
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：193
• 期：Complete
• 页码：362-367
• 全文大小：1493 K

文摘

Custard apple (Annona squamosa Linn.) is an edible tropical fruit, and its seeds have been used to treat “malignant sore” (cancer) and other usage as insecticide. A comparison of extraction processes, chemical composition analysis and antitumor activity of A. squamosa seed oil (ASO) were investigated.

Materials and methods

The optimal extraction parameters of ASO were established by comparing percolation, soxhlet, ultrasonic and SFE-CO₂ extraction methods. The chemical composition of fatty acid and content of total annonaceous acetogenins (ACGs) of ASO was investigated by GC-MS and colorimetric assay, and anti-tumor activity of ASO was tested using H₂₂ xenografts bearing mice.

Results

The optimal extraction parameters of ASO were obtained as follows: using soxhlet extraction method with extraction solvent of petroleum ether, temperature of 80 °C, and extraction time of 90 min. Under these conditions, the yield of ASO was 22.65%. GC-MS analysis results showed that the main chemical compositions of fatty acid of ASO were palmitic acid (9.92%), linoleic acid (20.49%), oleic acid (56.50%) and stearic acid (9.14%). The total ACGs content in ASO was 41.00 mg/g. ASO inhibited the growth of H₂₂ tumor cells in mice with a maximum inhibitory rate of 53.54% by oral administration. Furthermore, it was found that ASO exerted an antitumor effect via decreasing interleukin-6 (IL-6), janus kinase (Jak) and phosphorylated signal transducers and activators of transcription (p-Stat3) expression.

Discussion and conclusion

The results demonstrated that ASO suppressed the H₂₂ solid tumor development may due to its main chemical constituents unsaturated fatty acid and ACGs via IL-6/Jak/Stat3 pathway. ASO may be a potential candidate for the treatment of cancer.