Development of chemical isotope labeling liquid chromatography mass spectrometry for silkworm hemolymph metabolomics
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- 作者：Weifeng Shena ; b ; Wei Hanc ; Yunong Lic ; Zhiqi Mengb ; Leiming Caid ; cailm@mail.zaas.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Liang Lic ; Liang.Li@ualberta.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Mass spectrometry ; Liquid chromatography ; Isotope labeling ; Metabolomics ; Silkworm ; Pesticide
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：26 October 2016
- 年：2016
- 卷：942
- 期：Complete
- 页码：1-11
- 全文大小：2220 K
文摘
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A method was developed for profiling silkworm hemolymph metabolome with high coverage.
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Dansylation isotope labeling LC-MS was used for amine/phenol submetabolome quantification.
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Metabolomic changes in silkworm after DDT exposure were studied.
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Among 2044 metabolites detected, 65 were positively identified and 1809 were putatively identified.
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33 positively identified metabolites showed significant changes in five groups of silkworms.