Mining the human plasma proteome with three-dimensional strategies by high-resolution Quadrupole Orbitrap Mass Spectrometry
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- 作者：Yan Zhao1 ; Cheng Chang1 ; Peibin Qin ; Qichen Cao ; Fang Tian ; Jing Jiang ; Xianyu Li ; Wenfeng Yu ; Yunping Zhu ; zhuyunping@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Fuchu He ; hefc@bmi.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Wantao Ying ; proteomics@126.com" class="auth_mail" title="E-mail the corresponding author ; Xiaohong Qian ; qianxh1@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Plasma ; Proteome profiling ; IgY14 immunodepletion ; Biomarker
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：21 January 2016
- 年：2016
- 卷：904
- 期：Complete
- 页码：65-75
- 全文大小：1532 K
文摘
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Five three-dimensional strategies were developed and compared.
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The Strategy combined single Seppro IgY14 immunodepletion, high-pH RPLC fractionation and LC-MS/MS analysis was preferred as the optimum three-dimensional strategy.
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The optimal strategy generated 1544 plasma protein groups and 258 newly identified proteins.
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Up to 20 cytokines in the concentration range from sub-nanograms/milliliter to micrograms/milliliter were identified.