Ag nanoparticles dispersed on UF microsphere is used as an effective SERS substrate.
Excess formaldehyde in UF synthesis is crucial for preparation of the substrate.
Drying and incubation methods of the substrate affect the SERS performance.
Various inorganic salts modify the substrate basing on their affinity with silver.
Ag nanoparticles dispersed on UF microsphere has a R6G detection limit of 10−13 M.