Enhanced Performance of Osteoblasts by Silicon Incorporated Porous TiO₂ Coating

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• 作者：Quanming Wang^a ; Hongjie Hu^b ; Yuqing Qiao^b ; Zhengxiang Zhang^a ; Junying Sun^a ; ^{sun-junying@hotmail.com}
• 关键词：Micro-arc oxidation ; Silicon ; TiO2 ; Osteoblast ; Gene expression
• 刊名：Journal of Materials Science & Technology
• 出版年：2012
• 出版时间：February, 2012
• 年：2012
• 卷：28
• 期：2
• 页码：109-117
• 全文大小：1879 K

文摘

Silicon (Si) incorporated porous TiO₂ coating (Si-TiO₂) prepared on titanium (Ti) by micro-arc oxidation (MAO) technique was demonstrated to be cytocompatible in previous studies. In view of the potential clinical applications, a detailed in vitro study of the biological activity of Si-TiO₂ coating, in terms of osteoblast (MC3T3-E1 cells) morphology, proliferation, differentiation and mineralization was performed. Immunofluo-rescent staining indicated that cells seeded on the Si-TiO2 coating showed improved adhesion with developing mature cytoskeletons, which contained numerous distinct and well-defined actin stress fibers in the cell membranes compared with those on the TiO₂ coating and Ti plate. Results from proliferation assay showed that the proliferation rate of cells seeded on the Si-TiO₂coating was significantly faster than that on the TiO₂ coating and Ti plate. Furthermore, the analysis of osteogenic gene expression demonstrated that the Si-TiO₂ coating stimulated the expression of osteoblast-related genes and promoted differentiation and mineralization of MC3T3-E1 cells. In addition, the Si-TiO₂ coating differentially regulated Wnt signaling pathway by up-regulating the expression of low-density lipoprotein (LDL) receptor-related protein 5 (Lrp5), and down-regulating the expression of Dickkopf-1 (Dkkl). All together, these results indicate that the investigated titanium with Si-TiO₂ coating is biocompatible and a good candidate material used as implants.