Novel histone deacetylase inhibitors derived from Magnolia officinalis significantly enhance TRAIL-induced apoptosis in non-small cell lung cancer

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• 作者：Yuting Liu^a ; ^b ; ¹ ; Yunli Tong^a ; ^b ; ¹ ; Xia Yang^b ; Fangyuan Li^b ; Liang Zheng^a ; ^b ; Wenqin Liu^a ; ^b ; Jinjun Wu^b ; Rilan Ou^b ; Guiyu Zhang^b ; Ming Hu^b ; ^c ; Zhongqiu Liu^a ; ^b ; ^{liuzq@gzucm.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{liuzqsmu@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Linlin Lu^b ; ^{lllu@gzucm.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：NSCLC ; non-small cell lung cancer ; HDAC ; histone deacetylase ; HAT ; histone acetyltransferase ; PM ; polyphenol mixture ; DMSO ; dimethyl sulfoxide ; TV ; tumor volume ; SD ; standard deviation ; TSA ; trichostatin A
• 刊名：Pharmacological Research
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：111
• 期：Complete
• 页码：113-125
• 全文大小：6642 K

文摘

Histone modifications play critical roles in the progression of non-small cell lung cancer (NSCLC), which accounts for almost 85% of all diagnosed lung cancers. Magnolol and polyphenol mixture (PM) derived from Magnolia officinalis exhibited remarkable antitumor activities in lung cancer. However, the epigenetic effects and molecular mechanisms of magnolol and PM in NSCLC have yet to be reported. In this study, the epigenetic effects of magnolol and PM in NSCLC were examined in vitro and in vivo. Results revealed that magnolol and PM significantly suppressed the expression levels and function of class I histone deacetylases (HDACs). In A549 and H1299 cells, magnolol and PM remarkably induced cell apoptosis by arresting the cell cycle in the G0/G1 phase while simultaneously activating various pro-apoptotic signals, including TRAIL-R2 (DR5), Bax, caspase 3, cleaved caspase 3, and cleaved PARP. However, these apoptosis-promoting effects could be attenuated by TSA, which is a specific class I HDACs inhibitor. ChIP assays also demonstrated that magnolol and PM significantly enriched the histone acetyl mark (H3K27ac) in the promoter region of DR5. In A549 xenograft model, magnolol and PM notably reduced tumor growth by 44.40% and 35.40%, respectively. Therefore, magnolol and PM, as potential inhibitors of class I HDACs, induced tumor cell apoptosis and suppressed tumor growth partially by epigenetically activating DR5, which is a key protein in death receptor signaling pathway.