Polymorphism in a gene coding for the inflammasome component NALP3 and recurrent vulvovaginal candidiasis in women with vulvar vestibulitis syndrome

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• 作者：Ahinoam Lev-Sagie ; Diana Prus ; Iara M. Linhares ; Yuval Lavy ; William J. Ledger ; Steven S. Witkin
• 关键词：CIAS1 ; genetic polymorphism ; inflammasome ; interleukin-1β ; NALP3 ; recurrent vulvovaginal candidiasis ; vulvar vestibulitis syndrome
• 刊名：American Journal of Obstetrics and Gynecology
• 出版年：2009
• 出版时间：March 2009
• 年：2009
• 卷：200
• 期：3
• 页码：303.e1-303.e6
• 全文大小：735 K

文摘

Objective

Patients with vulvar vestibulitis syndrome (VVS) and control subjects were tested for a polymorphism in the gene coding for the NALP3 component of inflammasomes, cytoplasmic structures regulating interleukin (IL)-1β production.

Study Design

DNA from 143 women with VVS and 182 control women were tested for a length polymorphism in intron 4 of the gene (CIAS1) that codes for NALP3. Vestibular tissue was examined for NALP3 expression. Whole blood cultures were tested for Candida albicans-induced IL-1β production.

Results

The allele 12 frequency was higher in control subjects than in the patients with VVS (P = .02). Among patients with VVS and a self-reported history of recurrent vulvovaginal candidiasis (RVVC), the allele 7 frequency was 43.9 % as compared with 30.8 % in patients with no history of RVVC and 26.9 % in control women (P = .035 vs other patients and .001 vs control subjects). NALP3 was identified in vestibular tissue. C albicans-induced IL-1β production was reduced in samples from women with the 7,7 genotype (P = .030).

Conclusion

Polymorphism in the CIAS1 gene may play a central role in the triggering of VVS in a subset of patients.