Precise and accurate assay of pregnenolone and five other neurosteroids in monkey brain tissue by LC-MS/MS
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- 作者：Alain Y. Dury ; Yuyong Ke ; Fernand Labrie ; fernand.labrie@endoceutics.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Liquid chromatography&ndash ; tandem mass spectrometry ; Cynomolgus monkey ; Neurosteroids ; Girard T reagent
- 刊名：Steroids
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：113
- 期：Complete
- 页码：64-70
- 全文大小：1425 K
文摘
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There is a need for accurate quantitation of neurosteroids from various matrices.
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A highly sensitive and specific LC–MS/MS assay of six neurosteroids is proposed.
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The method proposed allows accurate measurements from Cynomolgus monkey brain tissue.
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The method is based on a Girard T ketone derivatization.