Sensitive detection of p53 tumor suppressor gene using an enzyme-based solid-state electrochemiluminescence sensing platform

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• 作者：Xiaoying Wang^a ; Xiangyi Zhang^a ; Pingang He^b ; ^{pghe@chem.ecnu.edu.cn} ; Yuzhi Fang^b ; ^{yuzhi@online.sh.cn}
• 关键词：MWNTs– ; Ru(bpy)₃²⁺ composite ; Solid-state electrochemiluminescence ; Glucose-dehydrogenase ; Wild type p53 sequence ; Muted type p53 sequence
• 刊名：Biosensors and Bioelectronics
• 出版年：2011
• 出版时间：15 April 2011
• 年：2011
• 卷：26
• 期：8
• 页码：3608-3613
• 全文大小：482 K

文摘

An enzyme-based solid-state electrochemiluminescence (ECL) sensing platform for sensitive detection of a single point mutation is developed successfully using p53 tumor suppressor gene as a model analyte. A composite of multiwalled carbon nanotubes and Ruthenium (II) tris-(bipyridine) (MWNTs–Ru(bpy)₃²⁺) was prepared and coated on an electrode surface, which was covered by polypyrrole (PPy) to immobilize ssDNA. Then, the ssDNA recognized the gold nanoparticle (AuNP)-labeled p53 tumor suppressor gene, and produced AuNP-dsDNA electrode with AuNP layer. The surface adsorbed the glucose-dehydrogenase (GDH) molecules for producing ECL signal. This system combined enzyme reaction with ECL detection, and it can recognize sequence-specific wild type p53 sequence (wtp53) and muted type p53 sequence (mtp53) with discrimination of up to 56.3 % . The analytic results were sensitive and specific. It holds promise for the diagnosis and management of cancer.