Apocynin and raisanberine alleviate intermittent hypoxia induced abnormal StAR and 3¦Â-HSD and low testosterone by suppressing endoplasmic reticulum stress and activated p66Shc in rat testes

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• 作者：Guo-Lin Zhang ; De-Zai Dai ; ^{dezaidai@vip.sina.com} ; Can Zhang ; Yin Dai
• 关键词：APO ; apocynin ; Bip ; immunoglobulin binding protein ; CHOP ; C/EBP homologous protein ; CPU86017-RS ; an optical isomer of CPU86017 ; a derivative of berberine ; RS ; raisanberine ; Cx43 ; connexin 43 ; CXs ; connexins ; DAPI ; 4&prime ; 6&prime ; diamidino-2-phenylindol
• 刊名：Reproductive Toxicology
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：36
• 期：Complete
• 页码：60-70
• 全文大小：1630 K

文摘

We hypothesized that hypoxia induced testicular damage is mediated by an activated NADPH oxidase (NOX), therefore, APO (apocynin) an inhibitor of NOX and raisanberine (RS), a calcium influx inhibitor were tested if they could attenuate hypoxic toxicity to the testis. Male Sprague-Dawley rats were exposed to hypoxia (10 ¡À 0.5 % O₂) for 17 d and intervened with APO and RS in the last 6 d. Histological changes and expression of pro-inflammation factors were evaluated in vivo. Biomarkers in isolated Leydig cells incubated with H₂O₂ were also assayed in vitro. Hypoxic rats displayed lower serum testosterone and higher LH and FSH. Upregulation of p22/p47^phox, NOX2, MMP9, PERK and p66Shc was associated with downregulation of StAR, 3¦Â-HSD and Cx43 in the hypoxia testis, revealed by Western blot and immunohistochemical assay, respectively. APO and RS at least partially normalize hypoxia caused male hypogonadism by suppressing ER stress, and p66Shc in testes.