Improved activity and thermo-stability of the horse radish peroxidase with graphene quantum dots and its application in fluorometric detection of hydrogen peroxide
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- 作者：Zhou Xiaoyana ; Jiang Yuanyuana ; Li Zaijuna ; b ; zaijunli@263.net" class="auth_mail" title="E-mail the corresponding author ; Gu Zhiguoa ; Wang Guanglia
- 关键词：Graphene quantum dots ; Horse radish peroxidase ; Hydrogen peroxide ; Colorimetric method
- 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
- 出版年：2016
- 出版时间：5 August 2016
- 年：2016
- 卷：165
- 期：Complete
- 页码：106-113
- 全文大小：1593 K
文摘
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We first reported horse radish peroxidase modification with graphene quantum dots.
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N,S-doped graphene quantum dots greatly improve the enzyme activity and stability.
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The enzyme performance depends on hydrophilic groups and structure of graphene quantum dots.
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The proposed method provides the advantage of sensitivity, stability and accuracy.
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The study provides a promising approach for adjusting enzyme activity and stability.