Crystal structure of the non-regulatory A4 isoform of spinach chloroplast glyceraldehyde-3-phosphate dehydrogenase complexed with NADP
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- 作者：Fermani ; Simona ; Ripamonti ; Alberto ; Sabatino ; Piera ; Zanotti ; Giuseppe ; Scagliarini ; Sandra ; et. al.
- 关键词：photosynthetic GAPDH ; NADP ; crystal structure ; cofactor selectivity ; spinach chloroplasts ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; rmsd ; root-mean-square deviation
- 刊名：Journal of Molecular Biology
- 出版年：2001
- 出版时间：November 30, 2001
- 年：2001
- 卷：314
- 期：3
- 页码：527-542
- 全文大小：1.07 M
文摘
Here, we report the first crystal structure of a photosynthetic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) complexed with NADP. The enzyme, purified from spinach chloroplasts, is constituted of a single type of subunit (A) arranged in homotetramers. It shows non-regulated NADP-dependent and NAD-dependent activities, with a preference for NADP. The structure has been solved to 3.0 Å resolution by molecular replacement. The crystals belong to space group C222 with three monomers in the asymmetric unit. One of the three monomers generates a tetramer using the space group 222 point symmetry and a very similar tetramer is generated by the other two monomers, related by a non-crystallographic symmetry, using a crystallographic 2-fold axis.