Adaptation to oxidative stress induced by polyunsaturated fatty acids in yeast
详细信息 查看全文
- 作者：Ana Cipak ; Morana Jaganjac ; Oksana Tehlivets ; Sepp D. Kohlwein ; Neven Zarkovic
- 关键词：Fatty acid peroxidation ; Reactive oxygen species ; Saccharomyces cerevisiae ; Fatty acid desaturase ; Catalase
- 刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids
- 出版年：2008
- 出版时间：June-July 2008
- 年：2008
- 卷：1781
- 期：6-7
- 页码：283-287
- 全文大小：353 K
文摘
To create a conditional system for molecular analysis of effects of polyunsaturated fatty acids (PUFA) on cellular physiology, we have constructed a strain of yeast (Saccharomyces cerevisiae) that functionally expresses, under defined conditions, the Δ12 desaturase gene from the tropical rubber tree, Hevea brasiliensis. This strain produces up to 15 % PUFA, exclusively under inducing conditions resulting in production of 4-hydroxy-2-nonenal, one of the major end products of n − 6 polyunsaturated fatty acid peroxidation. The PUFA-producing yeast was initially more sensitive to oxidative stress than the wild-type strain. However, over extended time of cultivation it became more resistant to hydrogen peroxide indicating adaptation to endogenous oxidative stress caused by the presence of PUFA. Indeed, PUFA-producing strain showed an increased concentration of endogenous ROS, while initially increased hydrogen peroxide sensitivity was followed by an increase in catalase activity and adaptation to oxidative stress. The deletion mutants constructed to be defective in the catalase activity lost the ability to adapt to oxidative stress.