Pathophysiological relevance of aldehydic protein modifications

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• 作者：Neven Zarkovic ; Ana Cipak ; Morana Jaganjac ; Suzana Borovic ; Kamelija Zarkovic
• 关键词：Non-enzymatic protein modifications ; Oxidative stress ; 4-Hydroxy-2-nonenal (HNE) ; Malondialdehyde (MDA) ; Acrolein (ACR) ; Immunochemistry
• 刊名：Journal of Proteomics
• 出版年：30 October, 2013
• 年：2013
• 卷：92
• 期：Complete
• 页码：239-247
• 全文大小：509 K

文摘

There is growing body of evidence that oxidative stress, i.e. excess in production of reactive oxygen species, can lead to covalent modification of proteins with bioactive aldehydes that are mostly produced under lipid peroxidation of polyunsaturated fatty acids. Thus generated reactive aldehydes are considered as second messengers of free radicals because they react with major bioactive macromolecules, in particular with various humoral and cellular proteins changing their structure and functions. Therefore, the aldehydic-protein adducts, in particular those involving 4-hydroxy-2-nonenal, malondialdehyde and acrolein can be valuable biomarkers of numerous pathophysiological processes. The development of immunochemical methods is increasing the possibilities to study such non-enzymatic protein modifications, on the one hand, while on the other hand the increase of knowledge on bioactivities of the aldehydes and their protein adducts might lead to better prevention, diagnosis and treatments of pathophysiological processes associated with lipid peroxidation and oxidative stress in general. This article is part of a Special Issue entitled: Posttranslational Protein modifications in biology and Medicine.