Effects of ZD7288 on firing pattern of thermosensitive neurons isolated from hypothalamus

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• 作者：Chunqing Cai¹ ; Xiaojing Meng¹ ; Junchu He ; Hangyu Wu ; Fei Zou ; ^{zoufei616@126.com}
• 关键词：HCN ; hyperpolarization-activated cyclic nucleotide-gated ; WSNs ; warm-sensitive neurons ; TINs ; temperature-insensitive neurons ; PO/AH ; the preoptic and anterior hypothalamus ; ISI ; interspike interval ; Ih ; hyperpolarization-activated current ; AP ; action pot
• 刊名：Neuroscience Letters
• 出版年：2012
• 出版时间：11 January 2012
• 年：2012
• 卷：506
• 期：2
• 页码：336-341
• 全文大小：849 K

文摘

The role of the hyperpolarization-activated current (Ih) mediated by HCN channels in temperature sensing by the hypothalamus was addressed. In warm-sensitive neurons (WSNs), exposure to ZD7288, an inhibitor of Ih mediated by hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, decreased their action potential amplitudes and frequencies significantly. By contrast, ZD7288 had little or no effect on temperature-insensitive neurons (TINs). Exposure of WSNs to ZD7288 led to a significant increase in the duration of the inter-spike interval and a reduction of Ih irreversibly. These results suggest that ZD7288 have the contrasting effects on the firing patterns of WSNs versus TINs, which implies HCN channels play a central role in temperature sensing by hypothalamic neurons.