Tethered Oligonucleotide-Primed Sequencing, TOP-Seq: A High-Resolution Economical Approach for DNA Epigenome Profiling

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• 作者：Zdislav Sta&scaron ; evskij¹ ; ³ ; Povilas Gibas¹ ; ³ ; Juozas Gordevičius¹ ; ² ; Edita Kriukienė¹ ; ^{edita.kriukiene@bti.vu.lt} ; Saulius Klima&scaron ; auskas¹ ; ⁴ ; ^{saulius.klimasauskas@bti.vu.lt}
• 关键词：mTAG labeling ; next-generation sequencing ; DNA unmethylome profiling ; neuroblastoma ; differential methylation ; DNA polymerase priming ; 5-methylcytosine ; CpG-specific methylation ; SssI methyltransferase
• 刊名：Molecular Cell
• 出版年：2017
• 出版时间：2 February 2017
• 年：2017
• 卷：65
• 期：3
• 页码：554-564.e6
• 全文大小：1751 K
• 卷排序：65

文摘

Chemo-enzymatic approach to map unmodified CpG sites at single-base resolution Demonstrates non-homologous proximity-driven priming of DNA polymerases Offers unidirectional DNA sequencing immediately from covalently tagged CpG sites Independent of bisulfite conversion and avoids whole-genome sequencing