Short- and long-term modulation of microvascular responses in streptozotocin-induced diabetic rats by glycosylated products

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• 作者：Maryam Bassirat ; Zeinab Khalil
• 刊名：Journal of Diabetes and its Complications
• 出版年：2010
• 出版时间：January-February 2010
• 年：2010
• 卷：24
• 期：1
• 页码：64-72
• 全文大小：669 K

文摘

Objective

This study aimed to determine the role of early and late glycation products in modulating inflammation in early diabetes.

Materials

Sprague–Dawley rats (130–170 g) were injected with streptozotocin (75 mg/kg, ip) and treated with daily aminoguanidine (AG, 25 mg/kg, ip) or vehicle for 2 or 4 weeks.

Methods

The base of a vacuum-induced blister raised on the hind paw was perfused with substance P (SP, 1 μM) and sodium nitroprusside (SNP, 100 μM). Changes in blood flow and plasma extravasation (PE) were measured. Amadori (1 mg/ml), advanced glycation end products (AGEs, 10 mg/ml), and anti-RAGE IgG (antibody against AGE receptors, 100 μg/ml) were individually perfused prior to SP.

Results

In diabetic rats, responses to SNP and SP were reduced by 60 % and 70 % , respectively (P<.05). Amadori increased responses to SNP by 50 % and 90 % and to SP by 70 % and 80 % in control and diabetic rats, respectively (both P<.05). SP responses were significantly increased after anti-RAGE IgG (70 % ) or AG treatments (175 % ) with PE responses normalized.

Conclusion

Amadori and anti-AGE agents enhance peripheral vascular responses in diabetes and may ameliorate microvascular damage.