Statistical analysis of the results showed that nucleation temperature of liquid samples with bubbles exhibited a better fit to linear equation, while samples without bubbles did not. The nucleation of samples containing bubbles occurred at temperatures close to the irradiation temperatures with a short delay less than 1 s, which was shorter than that without bubbles. In addition, the lagging time between the start of ultrasound irradiation to the occurrence of nucleation was shortened with the decrease in ultrasound irradiation temperatures. In addition, under the influence of ultrasound, water saturated with air bubbles nucleated at 鈭?#xA0;1 掳C with a shorter delay than water subsaturated with air bubbles, while nucleation of water subsaturated with air bubbles occurred after a shorter delay than water saturated with air bubbles at the temperature of 鈭?#xA0;2, 鈭?#xA0;3, 鈭?#xA0;4 and 鈭?#xA0;5 掳C, indicating that a number of pre-existing bubbles in liquid samples may also turn into cavitation bubbles similar to the ones generated by ultrasound. Results of the current study implied that the method of injecting bubbles into liquids before freezing could be used to initiate and control the nucleation process for liquid samples during ultrasound assisting freezing.
The study indicated that adding bubbles to liquid samples could be a promising and feasible approach to improve the effectiveness of ultrasound irradiation on the initiation of nucleation. This approach could have great potential for the frozen food industry.