Crucial residue Trp158 of lipoprotein PiaA stabilizes the ferrichrome-PiaA complex in Streptococcus pneumoniae

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• 作者：Liang Zhang¹ ; Nan Li¹ ; Kun Cao¹ ; Xiao-Yan Yang ; Guandi Zeng ; Xuesong Sun ; ^{tsunxs@jnu.edu.cn} ; Qing-Yu He ; ^{tqyhe@jnu.edu.cn}
• 关键词：Streptococcus pneumoniae ; Iron-binding protein ; PiaA ; Ferrichrome ; Iron transport
• 刊名：Journal of Inorganic Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：167
• 期：Complete
• 页码：150-156
• 全文大小：1172 K
• 卷排序：167

文摘

Pneumococcal iron acquisition ATP binding cassette is abbreviated to PiaABC. The cell-surface lipoprotein PiaA is a key component of PiaABC. Trp63, Trp158 and Phe255, in the metal binding site of PiaA, were mutated. Thermodynamic and kinetic study on the ferrichrome binding ability was performed. Trp158 is a crucial residue for structure stability and ferrichrome binding of PiaA.