Detection of the biotin–streptavidin interaction by exploiting surface stress changes on ultrathin Si membranes
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- 作者：Vasiliki Tsouti ; Christos Boutopoulos ; Peristera Andreakou ; Marina Ioannou ; Ioanna Zergioti ; Dimitris Goustouridis ; Dimitris Kafetzopoulos ; Dimitris Tsoukalas ; Pascal Norm ; Stavros Chatz ; roulis
- 关键词：Biosensor ; Capacitive readout ; Biotin– ; streptavidin
- 刊名：Microelectronic Engineering
- 出版年：2009
- 出版时间：April-June 2009
- 年：2009
- 卷：86
- 期：4-6
- 页码：1495-1498
- 全文大小：548 K
文摘
The detection of the biotin–streptavidin interaction is presented based on surface stress changes of functionalized Si membranes. During the biomolecular interaction, the surface stress of the functionalized membranes alters resulting in their deflection and finally in capacitance variations. The biological interaction on the sensors surface was verified after each experiment using fluorescent microscopy. Successful detection of 21 nM of streptavidin using 3.55 mM biotin probes is presented.