Combined Enzymatic Synthesis of Nucleotide (Deoxy) Sugars from Sucrose and Nucleoside Monophosphates

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• 作者：Zervosen ; Astrid ; Stein ; Andreas ; Adrian ; Holger ; Elling ; Lothar
• 刊名：Tetrahedron
• 出版年：1996
• 出版时间：February 12, 1996
• 年：1996
• 卷：52
• 期：7
• 页码：2395-2404
• 全文大小：503 K

文摘

The synthesis of NDP-glucose 3a-d (N= A, C, U, dU) with sucrose synthase B was combined with the enzymatic synthesis of nucleoside disphosphates 2a-d from their corresponding nucleoside monophosphates 1a-d by different kinases A. Further combination with recombinant dTDP-glucose 4,6-dehydratase D enabled us to synthesize dUDP-6-deoxy-α-D-xylo-4-hexulose 5 from 1d on a preparative scale. By using the repetitive batch technique the enzymatic syntheses of nucleotide (deoxy) sugars 3a-d, 5 could be realized on a 0.1 - 0.5 g scale.