Application of recombinant sucrose synthase-large scale synthesis of ADP-glucose

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• 作者：Zervosen ; Astrid ; Rö ; mer ; Ulrike ; Elling ; Lothar
• 关键词：ADP-glucose ; Sucrose synthase ; Nucleotide sugar
• 刊名：Journal of Molecular Catalysis B: Enzymatic
• 出版年：1998
• 出版时间：September 15, 1998
• 年：1998
• 卷：5
• 期：1-4
• 页码：25-28
• 全文大小：129 K

文摘

The synthesis of ADP-glucose with recombinant sucrose synthase from potato was combined with the synthesis of ADP from AMP and ATP catalysed by myokinase from rabbit muscle. By using the repetitive-batch-technique we were able to reach enzyme productivities (mg ADP-glucose/U enzyme) of 28 mg/U sucrose synthase and 140 mg/U myokinase yielding 2.8 g ADP-glucose (55 % yield). After product isolation, 2.2 g ADP-glucose was obtained corresponding to 44 % overall yield.