The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro
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- 作者：Pranapda Aumsuwana ; b ; Shabana I. Khana ; c ; Ikhlas A. Khana ; c ; Zulfiqar Alia ; Bharathi Avulaa ; Larry A. Walkera ; b ; d ; Zia Shariat-Madarb ; William G. Helferiche ; Benita S. Katzenellenbogenf ; Asok K. Dasmahapatraa ; b ; d ; asok@olemiss.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Dioscin ; GATA3 ; ZFPM2 ; Invasion/migration ; Gene expression
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：1 February 2016
- 年：2016
- 卷：591
- 期：Complete
- 页码：98-110
- 全文大小：1482 K
文摘
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Dioscin (DS), reduced cell viability of MCF-7 and MDA-MB-231 cells in a concentration-dependent manner.
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DS induced GATA3, DNMT3A, TET2, TET3 mRNA expression in MDA-MB-231 cells while TET1 was decreased in both cell lines.
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The cell morphology of MDA-MB-231 cells was altered by DS from spindle (mesenchymal) to cuboidal (epithelial) shape.
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GATA3 protein and mRNAs of ZFPM2 and E-cad were increased while VIM and MMP9 were decreased by DS.
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DS was able to inhibit invasion of MDA-MB-231 cells.