We designed and synthesized a benzothiazole-based fluorescent probe EPS-HS for selective detection of H2S. The probe EPS-HS showed good cell permeability and H2S could be detected within the cells. In situ visualization of H2S was performed on the hippocampal slices of normal mice with probe EPS-HS. The probe will be applied for better treatment of neurological deficits caused by damage to the hippocampus.