A putative silkworm Bombyx mori APH gene was cloned and sequenced in insects.
Heterologous expression of BmAPH gene in E. coli showed that BmAPH has acylpeptide hydrolase activity toward the traditional substrate, Ac–Ala–pNA.
The enzyme activity of BmAPH was inhibited by the OP compounds in vitro and in vivo, suggesting that it may be involved in OP insecticides resistance of silkworm.
Immunohistochemistry analysis showed that BmAPH protein was localized in the basement membranes in head and midgut.