A novel alkaline surfactant-stable keratinase with superior feather-degrading potential based on library screening strategy
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- 作者：Chang Su ; Jin-Song Gong ; Rong-Xian Zhang ; Li-Yan Tao ; Wen-Fang Dou ; Dan-Dan Zhang ; Heng Li ; Zhen-Ming Lu ; Zheng-Hong Xu ; Jin-Song Shi ; shijs@163.com
- 关键词：Keratinase ; Fosmid library ; Characterization
- 刊名：International Journal of Biological Macromolecules
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：95
- 期：Complete
- 页码：404-411
- 全文大小：1357 K
- 卷排序：95
文摘
A novel keratinase coding gene was mined via function-driven screening with fosmid library. The keratinase gene was successfully expressed in Escherichia coli BL21 (DE3). Recombinant keratinase with excellent tolerance towards various surfactants was purified and characterized. The enzyme displayed promising application potential in biodegradation of keratin wastes.