A stented excisional wound model was established, and the wound healing among vehicle control, TRH, and TRH analog taltirelin treatment groups was evaluated by macroscopic and histologic analyses. Primary fibroblasts were isolated from rat dermis and treated with vehicle control, TRH or taltirelin, cell migration, and proliferation were examined by scratch migration assay, MTT, and 5-ethynyl-2′- deoxyuridine (EdU) assay. The expression of 伪-Smooth muscle actin in fibroblasts was detected by Western blot and immunocytochemical analysis.
TRH or taltirelin-treated wounds exhibited accelerated wound healing with enhanced granulation tissue formation and increased re-epithelialization and tissue formation. Furthermore, TRH or taltirelin promoted the migration and proliferation of fibroblasts and induced the expression of 伪-Smooth muscle actin in fibroblasts.
TRH is important in upregulating the phenotypes of dermal fibroblasts and plays a role in accelerating wound healing.