Effects of Focused Extracorporeal Shock Waves on Bone Marrow Mesenchymal Stem Cells in Patients with Avascular Necrosis of the Femoral Head

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• 作者：Lei Zhai^&lowast ; ; ^&dagger ; ; Nan Sun^&Dagger ; ; Bo Zhang^§ ; ; Shui-Tao Liu^&dagger ; ; Zhe Zhao^¶ ; ; Hai-Chao Jin^&dagger ; ; Xin-Long Ma^‖ ; Geng-Yan Xing^¶ ; ; ^{zhangbomianyi@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Extracorporeal shock wave ; Femoral head ; Bone marrow mesenchymal stem cells
• 刊名：Ultrasound in Medicine & Biology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：42
• 期：3
• 页码：753-762
• 全文大小：1567 K

文摘

To observe the effect of extracorporeal shock waves (ESWs) on bone marrow mesenchymal stem cells (MSCs) in patients with avascular necrosis of the femoral head, we collected bone marrow donated by patients and then cultivated and passaged MSCs in vitro using density gradient centrifugation combined with adherence screening methods. The P3 generation MSCs were divided into the ESW group and the control group. The cell counting kit for MSCs detected some proliferation differences. Cytochemistry, alkaline phosphatase staining and Alizarin red staining were used to determine alkaline phosphatase content. Simultaneously, real-time polymerase factor α1, osteocalcin and peroxisome proliferator-activated receptor γ. Together, the results of our study first indicate that moderate ESW intensity, which is instrumental in enhancing MSC proliferation, inducing conversion of MSCs into osteoblasts, and inhibiting differentiation of MSCs into adipocytes from MSCs, is one of the effective mechanisms for treating avascular necrosis of the femoral head.