Multiple approaches to assess pectin binding to galectin-3
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- 作者：Tao Zhang ; Yi Zheng ; Dongyang Zhao ; Jingmin Yan ; Chongliang Sun ; Yifa Zhou ; zhouyf383@nenu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Guihua Tai ; taigh477@nenu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：ASF ; asialofetuin ; FITC-Lac ; 2-(fluorescein-5-thiourea)-ethyl(β-d-galactopyranosyl)-(1 ; &rarr ; ; 4)-β-d-glucopyranoside) ; BLI ; bio-layer interferometry ; cFLISA ; competitive fluorescence-linked immunosorbent assay ; FP ; fluorescence polarization ; Gal-3 ; galectin-3 ; G3H ; Gal-3-mediated hemagglutination ; HPLC ; high performance liquid chromatography ; MCP ; pH-modified citrus pectin ; SPR ; surface plasmon resonance
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：91
- 期：Complete
- 页码：994-1001
- 全文大小：985 K
文摘
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Gal-3 and pectin interactions were evaluated using five different methods.
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Results obtained from these methods were generally in agreement.
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Binding/inhibition parameters (KD, IC50, and MIC) differ to some extent.
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Data derived from direct binding assays were comparable.
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Data derived from inhibition assays depended greatly on the probes used.